FastQCFastQC Report
Thu 26 May 2016
ERR489025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19457185
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223900.11507317219834215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328250.039.8096051
GTATCAA619850.032.8491671
TATCAAC797400.025.280552
TCAACGC795450.025.2621464
ATCAACG802550.025.0118313
CAACGCA812700.024.7439235
AACGCAG848200.023.7811246
GTACCTG222900.022.33481
TACCTGG232500.020.8916662
ACGCAGA968750.020.7262887
GAGTACT565600.020.31441312
CGCAGAG991650.020.2390128
AGTACTT584100.019.63074513
GTACATG785850.019.5707321
AGAGTAC994500.019.47889111
GTACTTT597900.019.009614
TACATGG807200.018.7848262
CAGAGTA1057300.018.6511110
GCAGAGT1073400.018.5158189
GTGGTAT148250.018.3830661
Produced by FastQC (version 0.11.5)