FastQCFastQC Report
Thu 26 May 2016
ERR489025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19457185
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224990.1156333765650067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA330550.039.5010341
GTATCAA611000.032.1569181
TCAACGC787300.024.7823414
TATCAAC796000.024.5945912
ATCAACG793600.024.5689743
CAACGCA805800.024.2305015
AACGCAG842250.023.2760986
GTACCTG233250.021.9079911
TACCTGG237150.020.9945492
GAGTACT567800.020.24382612
ACGCAGA972100.020.1277877
CGCAGAG997750.019.5863938
AGTACTT586900.019.4321913
AGAGTAC1005950.018.96407711
GTACATG780350.018.8560051
GTACTTT603100.018.76721814
GTGGTAT152650.018.1316341
CAGAGTA1073000.018.0940710
TACATGG801900.018.0671232
GCAGAGT1094050.017.824769
Produced by FastQC (version 0.11.5)