FastQCFastQC Report
Thu 26 May 2016
ERR489024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14215363
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1490031.0481828708841272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218670.1538265326041973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162710.11446067188013419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG176800.025.835552
GTACCTG189750.024.5342041
ACCTGGG194350.022.5597673
TATAACG9500.022.532442
GAGTACT497950.020.61674512
AGTACTT505150.020.24226613
GTACATG747250.019.8222981
TACATGG750350.019.3942872
GTACTTT530050.019.15546814
AGAGTAC933150.018.84298511
ACATGGG767500.018.5325093
TACTTTT571550.017.74106815
CCTGGGG145150.017.649284
GAGTACG23800.017.557891
ACTTTTT592950.017.5515116
CATGGGG441850.016.9248074
TACACGG20700.015.6782532
CATGGGT118750.015.6418764
ATGGGGG258500.015.6094435
GTGTAGC64600.015.3965651
Produced by FastQC (version 0.11.5)