FastQCFastQC Report
Thu 26 May 2016
ERR489024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14215363
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1497561.053479956860757No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA347940.2447633591910386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210920.1483746844874802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163490.11500937401317153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG181550.024.5936412
GTACCTG190050.024.1045191
ACCTGGG192100.022.271613
GAGTACG22400.020.065311
GAGTACT515450.019.3226712
AGTACTT517700.019.28574613
GTACATG763250.018.6403271
TATAACG8100.018.3175872
GTACTTT540350.018.2730314
TACATGG765550.018.231812
AGAGTAC973300.017.68017811
ACATGGG779950.017.354373
TACTTTT581250.016.96271715
ACTTTTT603600.016.44893516
CCTGGGG145400.016.4209774
TACGCAG22650.016.2994484
CATGGGG445100.016.0228654
TAGATCG6650.015.0460145
GTGTAGC67700.014.7590921
ATGGGGG260100.014.71085
Produced by FastQC (version 0.11.5)