FastQCFastQC Report
Thu 26 May 2016
ERR489023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12780764
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCT118000.021.461884
GTACCTG112000.021.2483441
GTACCCG7950.020.539191
GTATAGC75650.020.2985921
GTGTAGC86650.019.926931
TACCTGG117350.019.546042
GAGTACT309200.018.98705112
AGTACTT316800.018.54353113
GTAGCCC76500.018.2686753
GTCCTAC70100.018.0399761
ATAGCCC70400.017.836973
GTATCAA622550.017.4950791
GTACTTT336800.017.39105214
GTACATG511300.017.265591
TACATGG510350.016.8591632
CGACTTG36350.016.6112264
TTGACGA35500.016.52357160
CTTGACG36050.016.36686959
GTAGGAC83050.016.2863673
TATAGCC80600.016.2679392
Produced by FastQC (version 0.11.5)