FastQCFastQC Report
Thu 26 May 2016
ERR489023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12780764
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2451.8189894E-1221.12057350
TAGCCCT124100.021.018594
GTACCCG8450.020.4697251
GTGTAGC96900.019.5282021
GTACCTG115200.019.4290031
CGACTTG36850.019.19502364
GTATAGC77150.018.653321
CGACGAG3700.018.64699224
GTAGCCC84050.018.5138113
GTCCTAC68250.018.4500451
GAGTACT325600.018.42543612
TTGACGA36900.018.32744860
TACCTGG120350.018.2620832
GTAGGAC82450.017.9942933
AGTACTT334550.017.88095313
GACGACT38300.017.74760462
ACGACTT39050.017.5834663
TGACGAC39200.017.25211361
CTTGACG38250.017.22955959
GTACTTT353350.016.8807814
Produced by FastQC (version 0.11.5)