FastQCFastQC Report
Thu 26 May 2016
ERR489022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380311
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1208450.7377454555044772No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA359570.21951353670879634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199530.1218108740426235No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC196030.11967416247469294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG203550.027.9751361
TACCTGG203800.027.4588132
ACCTGGG239100.022.9213053
TATAACG9950.020.148562
GAGTACT479850.019.95361112
AGTACTT494350.019.30640613
AGAGTAC841350.018.87503211
GTACATG606800.018.6539781
GTACTTT513550.018.47677614
TACATGG616200.017.9949972
CCTGGGG205450.017.742624
GTACCCG30050.017.5629961
TACTTTT555250.017.12849815
ACATGGG643750.016.769093
ACTTTTT582800.016.59006516
TACCCGG31200.016.0639572
GAGTACG26550.015.9549261
GTACACG32250.015.503591
CATGGGG398300.014.8946894
TACACGG27200.014.4868862
Produced by FastQC (version 0.11.5)