FastQCFastQC Report
Thu 26 May 2016
ERR489022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380311
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1245110.7601259829560013No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA293650.17927010054937298No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC215860.131780159729568No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC206410.12601103849615553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199360.12170709090932401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG204550.027.1445641
TACCTGG201750.027.0722832
ACCTGGG239450.022.219133
TATAACG11400.020.278112
GAGTACT502450.019.60809512
AGTACTT516900.018.91318513
GAGTACG25250.018.633091
AGAGTAC859200.018.42040411
GTACTTT533950.018.2963314
GTACATG593750.017.8871881
ATAACGC15050.017.881983
TACATGG604700.017.3513772
CCTGGGG200950.017.1174134
TACTTTT571600.017.10893215
TAACGCA15800.017.0321124
ACTTTTT599800.016.39651316
TATCACG7850.016.2625852
ACATGGG628200.016.2574083
TACGCAG26150.015.3044364
GTATAGG29500.015.2450431
Produced by FastQC (version 0.11.5)