FastQCFastQC Report
Thu 26 May 2016
ERR489021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28850876
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA454700.038.065781
GTATCAA853400.031.7701341
TCAACGC1082900.024.7418334
ATCAACG1085150.024.7124843
TATCAAC1093950.024.6026672
CAACGCA1097950.024.3668885
AACGCAG1144550.023.4944676
ACGCAGA1311600.020.4244567
CGCAGAG1325350.020.148358
GAGTACT792900.020.12593712
GTACCTG300150.019.9901811
AGTACTT825800.019.22573713
AGAGTAC1321650.019.18028511
TACCTGG306650.019.1207052
GTACTTT856150.018.53452514
GTACATG944650.018.3958871
CAGAGTA1414200.018.32721110
GCAGAGT1446650.018.0400169
TACATGG973400.017.4883062
GTGGTAT233850.017.3614641
Produced by FastQC (version 0.11.5)