FastQCFastQC Report
Thu 26 May 2016
ERR489021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28850876
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA466200.039.0050351
GTATCAA844550.030.2395291
ATCAACG1076300.023.4260033
TCAACGC1077500.023.3961854
TATCAAC1087950.023.3051742
CAACGCA1097250.022.9838985
AACGCAG1145500.022.1243636
ACGCAGA1324850.019.0998977
CGCAGAG1337950.018.8972878
GAGTACT819250.018.81861912
GTACCTG301550.018.1342951
AGAGTAC1354500.018.12386311
AGTACTT851050.018.05923313
GTACTTT871000.017.58617814
TACCTGG302200.017.5359692
GTGGTAT237450.017.5054661
GTACATG951850.017.3368661
CAGAGTA1447150.017.278210
TGGTATC228250.016.9898092
GCAGAGT1482800.016.9605069
Produced by FastQC (version 0.11.5)