FastQCFastQC Report
Thu 26 May 2016
ERR489020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22470189
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290270.1291800438349673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG21150.025.6551341
GTACCTG243900.025.1944561
TACCTGG244000.024.480542
TACCCGG22150.023.0969752
ACCTGGG254550.022.4449673
GTGTAGC125200.020.2065321
GAGTACT772000.020.05540312
AGTACTT782650.019.76611713
TAGCCCT174950.019.5190334
GTATAGC112850.019.3553281
GAGTACG32000.019.3325041
GTACTTT829000.018.59109514
GTACATG1092450.018.2856881
TACATGG1087100.017.959422
AGAGTAC1429950.017.95312311
ATAGCCC94650.017.8934763
GTCCTAC124400.017.5860541
ACATGGG1086700.017.3565563
GTAGGAC141650.017.2757113
GTAGCCC118100.017.237913
Produced by FastQC (version 0.11.5)