FastQCFastQC Report
Thu 26 May 2016
ERR489020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22470189
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA689790.30698006144941636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264220.11758690592233113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG239800.023.3435381
GTACCCG20300.022.8373681
TACCTGG240600.022.8282222
ACCTGGG250350.020.8091813
TACCCGG22750.019.2595862
TAGCCCT178500.018.5350784
GTGTAGC125900.018.4663431
GAGTACT789000.018.4558912
GTAGGAC151600.018.3426253
AGTACTT801900.018.1288813
CGACTTG87250.017.39989764
GACGACT89300.017.38683362
TTGACGA87800.017.36725460
GTACTTT835550.017.28316914
GTCCTAC116800.017.1799721
GTACATG1069000.017.1172181
GTATAGC111250.017.104091
TACATGG1064700.016.8305912
CTTGACG89400.016.70909159
ACGACTT93100.016.64010663
Produced by FastQC (version 0.11.5)