FastQCFastQC Report
Thu 26 May 2016
ERR489019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14835007
Sequences flagged as poor quality0
Sequence length75
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5883523.9659704912845677No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1750621.180060110521013No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1362660.9185435504007513No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1252590.8443474276756324No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1031190.6951058398556873No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1009530.6805052400716765No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1008420.6797570098888392No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG496370.3345937079773538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG189750.031.6257762
GTACCTG202400.030.0410751
GCTTATG45550.028.5500281
TTATGTA44100.028.5291423
GAGTACG75450.028.0542891
CTTATGT46500.027.4313182
TATAACG5800.026.8199392
ACCTGGG228750.025.9287823
TACGCAG77700.025.842984
TATGTAC50700.025.1539334
AGTACGC84550.024.40812
GTACGCA83550.024.366593
ACGCAGT87150.023.04125
TAGATCG7700.022.4427265
CGCAGTG98350.020.43016
TACCCGG24400.019.8340552
CCTGGGG197750.019.3297444
GAGTAGG62250.018.279471
GTACCCG27700.018.1048661
TACTTTT258050.018.07344615
Produced by FastQC (version 0.11.5)