FastQCFastQC Report
Thu 26 May 2016
ERR489019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14835007
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5933363.9995667005752003No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA1739191.172355361881528No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1444020.9733868005589751No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1349520.9096861228309497No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1087850.7332992832426706No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1086080.7321061594376059No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1057410.7127802501205426No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG308260.20779228483006446No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAC150260.10128744799378928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG196000.031.1165962
GAGTACG75950.030.1852761
GTACCTG205350.030.1585371
GCTTATG46200.029.8634741
TTATGTA44950.029.240193
TACGCAG76250.029.0908244
TATAACG6800.027.3937022
CTTATGT49350.027.1911962
GTACGCA84700.026.514443
AGTACGC85700.026.4051672
ACGCAGT85750.025.8675865
ACCTGGG232250.025.8451123
TATGTAC52900.025.4327814
CGCAGTG99300.022.4072996
CCTGGGG201200.019.683344
GTATACG4950.018.8611411
TACACGG20450.018.5551832
GAGTAGG61500.018.3295521
GCTTATA13600.018.0521471
TATCACG8500.017.8566342
Produced by FastQC (version 0.11.5)