FastQCFastQC Report
Thu 26 May 2016
ERR489018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22432600
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494980.22065208669525602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347110.15473462728350704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239870.10692920125174969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA852000.026.9535031
GTACCTG300250.026.546141
GTATCAA1350800.026.2326811
TACCTGG302500.025.8052982
ACCTGGG340150.022.2247433
GAGTACT909750.021.6089312
TATCAAC1645050.021.3912582
AGTACTT932500.021.06059613
ATCAACG1670350.021.058533
TCAACGC1671050.021.0274
CAACGCA1694350.020.7217755
GTACTTT964700.020.24736414
AACGCAG1738900.020.24216
GTACATG1281850.019.8807221
TATAACG16000.019.662182
AGAGTAC1696450.019.43443711
TACATGG1300500.019.1900882
TACTTTT1040400.018.79222115
ACTTTTT1080550.018.50895516
ACATGGG1321150.018.314893
Produced by FastQC (version 0.11.5)