FastQCFastQC Report
Thu 26 May 2016
ERR489018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22432600
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467160.20825049258668188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348100.15517594928808964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253240.11288927721262805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA852800.026.7100261
GTACCTG298600.025.5167371
TACCTGG295550.025.3662552
GTATCAA1320350.025.0245591
ACCTGGG331100.021.6634063
TATCAAC1630250.020.127952
GAGTACT904600.020.02566312
ATCAACG1649300.019.8433463
TCAACGC1655800.019.766434
AGTACTT929100.019.61619413
CAACGCA1682150.019.4465035
TATAACG17500.019.123232
AACGCAG1728250.018.9858386
GTACTTT952600.018.83169414
GTACATG1286250.018.5484031
TACATGG1296650.018.1383652
AGAGTAC1718100.018.06147611
TACTTTT1023150.017.51263415
TACCCGG36350.017.3689792
ACATGGG1310150.017.2722153
Produced by FastQC (version 0.11.5)