FastQCFastQC Report
Thu 26 May 2016
ERR489017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26534228
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7060562.660925352718006No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2107030.7940800086590045No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1532260.5774654533005446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG79700.026.9964621
GTACCTG267250.024.9812621
TACCTGG270050.024.2077792
TACGCAG87550.023.2527374
ACCTGGG294500.021.2675173
ACGCAGT96950.020.9986575
GCTTATG75900.019.9165651
GTACGCA104600.019.8933853
AGTACGC107650.019.6216142
GAGTACT579000.019.53499412
AGTACTT602750.018.78285213
GTACTTT597900.018.6429414
GTACATG920650.018.2681751
CGCAGTG115600.017.7403016
TACATGG949050.017.484762
TACCCGG33550.017.2080422
AGAGTAC1187000.017.19974111
TACTTTT667600.016.7357615
ACATGGG976700.016.5626013
ACTTTTT690650.016.44066616
Produced by FastQC (version 0.11.5)