FastQCFastQC Report
Thu 26 May 2016
ERR489017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26534228
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1352040.5095456329085587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG80900.024.1938361
GTACCTG267100.023.3819481
TACCTGG269600.022.7244742
GCTTATG82250.021.6104621
TACGCAG87300.021.3778694
ACGCAGT93500.020.070535
ACCTGGG299150.019.661693
GAGTACT590550.018.46332612
AGTACTT609350.017.89934713
GTACTTT608900.017.7029714
GTACGCA108550.017.7015323
AGTACGC108900.017.6757182
GTACATG945200.017.272181
CGCAGTG109450.016.9565416
TACCCGG32800.016.511882
TACATGG984750.016.4537642
CTTATGT104800.016.4251752
TTATGTA104500.016.4068643
GTATAGG55300.016.0085371
AGAGTAC1238800.015.98549211
Produced by FastQC (version 0.11.5)