FastQCFastQC Report
Thu 26 May 2016
ERR489016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18101584
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7571284.182661583649254No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2179841.2042261052955365No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1610670.8897950588191619No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1539460.8504559600971937No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1319670.7290356468251619No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1209550.6682011916747176No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG601360.33221402060725735No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC232170.1282594937548007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181490.1002619439271171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG89650.030.9345861
TACGCAG97450.027.7712144
AGTACGC104950.026.0570722
GTACGCA106350.025.612663
GCTTATG58250.025.2297651
ACGCAGT107550.024.9328985
GTACCTG156950.024.7862851
TACCTGG158700.024.0069082
CGCAGTG115000.023.2542296
ACCTGGG178500.020.4305573
CTTATGT71500.019.9215322
GAGTACT443400.018.36329712
AGTACTT450950.018.04986613
GTACATG564400.017.4858681
GTACTTT472000.017.18549714
TACATGG573900.016.8676012
TATGTAC86150.016.6497544
TTATGTA85000.016.3076523
TACTTTT503550.016.05370115
ACATGGG591150.016.0337733
Produced by FastQC (version 0.11.5)