FastQCFastQC Report
Thu 26 May 2016
ERR489016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18101584
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7879894.353149426039179No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2222851.227986456875818No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1758560.971495091258312No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1691670.9345425240133681No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA1437200.7939636663841131No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC1341110.7408799141555789No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1297270.7166610391665171No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG881950.4872225546670391No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG662050.3657414732323978No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG359150.19840805091974273No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA202920.11210068687911512No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAC183450.10134472209724851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG86200.031.369591
TACGCAG92750.028.4919644
GCTTATG58350.027.3542731
ACGCAGT99900.026.245915
AGTACGC104750.025.3267732
GTACGCA106450.024.9871223
CGCAGTG106900.024.5268766
TACCTGG157700.024.3265572
GTACCTG161250.023.9408871
CTTATGT75600.020.9002362
ACCTGGG179700.020.2732493
TATGTAC91750.017.6349544
TTATGTA89250.017.5104583
GAGTACT462550.017.28052312
AGTACTT469500.017.0690913
GTACATG583200.016.4269431
GTACTTT485450.016.32349814
GAGTAGG61150.015.9667951
TACATGG591850.015.9365522
TACTTTT510600.015.51925615
Produced by FastQC (version 0.11.5)