FastQCFastQC Report
Thu 26 May 2016
ERR489015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13926524
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA534040.38346970141292974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243230.17465233966494437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173720.12474038747931646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG182700.027.160362
GTACCTG188250.026.58721
ACCTGGG190750.024.9240863
GTACCCG16450.021.65761
GAGTACT533000.020.55360412
AGTACTT542350.020.27043213
GTACTTT571450.019.12239814
TACCCGG18950.018.4303822
AGAGTAC937450.018.42915511
GTACATG696150.018.4186941
TACATGG698950.018.112392
GTGTAGC71350.017.7429641
ATAACGC12600.017.562673
TACTTTT629600.017.41068615
ACATGGG701400.017.3079723
ACTTTTT656400.017.18952816
GTCCTAC90450.017.1320171
GTAGGAC100100.016.8564783
TAGCCCT98150.016.7668694
GTATAGC60850.016.7118991
Produced by FastQC (version 0.11.5)