FastQCFastQC Report
Thu 26 May 2016
ERR489015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13926524
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232760.1671343114764316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182180.13081512658865915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG182450.027.4935872
GTACCTG191850.026.6788481
ACCTGGG189950.025.245643
GTACCCG16450.019.5781441
GAGTACT550750.018.62839912
TATAACG11950.018.4766392
AGTACTT559800.018.38926313
TAACGCA12550.017.5932924
GTACTTT582200.017.5513814
GTACATG707150.017.3456841
TACCCGG18500.017.1564332
AGAGTAC974750.017.11538911
TACATGG711450.017.0593452
GTAGGAC101750.016.681753
ATAACGC13650.016.6810043
GTCCTAC89750.016.6301921
GTGTAGC80450.016.572541
ACATGGG713450.016.3345453
CCTGGGG142050.016.1749864
TAGGACC48800.016.1185474
Produced by FastQC (version 0.11.5)