FastQCFastQC Report
Thu 26 May 2016
ERR489014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18592424
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243190.13080058845473833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG208900.024.9271661
TACCTGG205400.024.8090532
GAGTACT566400.020.55375912
AGTACTT574700.020.39019413
ACCTGGG244350.020.3811043
GTACTTT595900.019.38126614
GTACATG931600.019.35871
TACATGG948900.018.6681732
TAGCCCT113600.018.5867024
AGAGTAC1108350.018.56423411
GTGTAGC93400.018.3239941
ACATGGG946850.018.0566353
TACTTTT656700.017.64317915
ACTTTTT685200.017.1377416
TAGATCG16600.017.0685585
GTATAGC76150.016.2749041
GTCCTAC70700.016.1546131
CATGGGG495150.015.8775724
TATAACG11600.015.4973872
GTATCAA1564350.015.2965831
Produced by FastQC (version 0.11.5)