FastQCFastQC Report
Thu 26 May 2016
ERR489014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18592424
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242880.13063385387510526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG203850.024.6760851
TACCTGG203200.024.280332
GAGTACT565150.020.40604612
AGTACTT577000.020.05283713
ACCTGGG243650.019.626333
GTACTTT589850.019.3761914
GTACATG934000.018.9683881
GTGTAGC99900.018.7350271
TAGCCCT118350.018.5984694
TACATGG944450.018.3240222
AGAGTAC1114650.018.27852411
TACCCGG23850.018.227392
ACATGGG946450.017.6874563
TACTTTT656550.017.46496615
ACTTTTT678650.017.02321216
TATAACG13400.016.2210162
GTATAGC78550.015.9875891
CATGGGG490050.015.9741914
GTAGGAC78350.015.8527933
TAGATCG15350.015.7331055
Produced by FastQC (version 0.11.5)