FastQCFastQC Report
Thu 26 May 2016
ERR489013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14901933
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1196270.802761628306878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307180.20613433170045792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224890.15091330768968028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156060.10472466894059985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT589700.021.09760512
AGTACTT600450.020.76141413
GTACTTT617200.019.99634714
GTACATG944650.019.6417961
TACATGG955450.019.0778222
AGAGTAC1158050.018.70027511
TACTTTT666600.018.54658715
ACATGGG964400.018.3565353
GTACCTG148200.018.3367651
ACTTTTT686650.018.23705716
CATGGGT147350.017.8225464
TACCTGG154400.017.2787342
CGCGTTC12850.017.18491660
GTATAGG33600.016.4026011
CATGGGG481550.015.9936944
ATGGGTA87650.015.4138685
ACCTGGG162050.015.3711693
GTGTAGC87850.014.9932821
CTTTTTT839750.014.97719217
GTACACG26400.014.9677251
Produced by FastQC (version 0.11.5)