FastQCFastQC Report
Thu 26 May 2016
ERR489013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14901933
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314440.2110061828891594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238780.16023424612095627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166410.11167007662697183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT620700.020.56414212
AGTACTT632000.020.16916813
GTACTTT644100.019.51176314
CGCGTTC11700.019.16972260
GTACATG960850.019.0565281
GAGTACG19450.019.0310021
TACATGG971850.018.603042
TACTTTT693750.018.12966315
AGAGTAC1207850.018.0507311
GTACCTG148600.017.8788621
ACTTTTT708250.017.8559116
ACATGGG977400.017.8302293
TACCTGG150000.017.4583172
GTATAGG31500.016.3633861
CATGGGT153500.015.9140114
CATGGGG481100.015.727444
ATGGGCG32650.015.5342015
CATGGGC232700.015.4647434
ACCTGGG163900.014.9462173
CGCAATA13250.014.8400436
Produced by FastQC (version 0.11.5)