FastQCFastQC Report
Thu 26 May 2016
ERR489012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18695047
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1004450.024.2251
GGTATCA663450.024.0448271
TACCTGG263650.021.7212752
GTACCTG278850.020.9516181
GAGTACT565000.020.6776412
AGTACTT576250.020.14943513
TCAACGC1225950.019.626944
TATCAAC1234850.019.55982
ATCAACG1232250.019.5426623
GTACATG976750.019.5280131
GTACTTT593000.019.51737614
CAACGCA1246800.019.3000285
AACGCAG1280750.018.8622236
AGAGTAC1197550.018.79969611
TACATGG1007000.018.692732
ACCTGGG292200.018.4625873
TACTTTT632150.018.2767115
ACTTTTT661350.017.79655816
ACATGGG1028950.017.7315483
ACGCAGA1415250.016.9964947
Produced by FastQC (version 0.11.5)