FastQCFastQC Report
Thu 26 May 2016
ERR489012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18695047
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA683750.023.6246831
GTATCAA1011350.023.3477761
TACCTGG262600.021.3521752
GTACCTG277950.020.9023971
GAGTACT586550.019.69177612
AGTACTT592750.019.39273513
TCAACGC1238650.018.8028434
GTACATG992450.018.7224061
ATCAACG1246550.018.7113573
TATCAAC1257700.018.6201932
GTACTTT610950.018.61174814
CAACGCA1259850.018.4938145
ACCTGGG287850.018.243863
AGAGTAC1232650.018.17790411
AACGCAG1298950.018.0275386
TACATGG1019700.017.9445532
TACTTTT648900.017.51710515
ACATGGG1035200.017.0419733
ACTTTTT671450.017.0418416
TATAACG11550.016.7297522
Produced by FastQC (version 0.11.5)