FastQCFastQC Report
Thu 26 May 2016
ERR489011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16838366
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258040.15324527332402682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183240.10882291072661088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG306100.034.6964531
TACCTGG304650.034.3790442
ACCTGGG347350.029.5111263
TATAACG16000.024.412422
CCTGGGG264100.023.4768524
TACACGG36200.020.911632
GTACACG41950.020.678911
TATCACG12250.020.5986672
TAACGCA19550.020.3299794
GAGTACT583000.020.24364512
GTACCCG33250.020.1648921
AGTACTT598100.019.7853913
TACCCGG32950.019.0927412
GTACATG718250.019.0644661
AGAGTAC1013200.019.05476811
ATAACGC23000.018.9332263
GTACTTT636700.018.4190714
TACATGG736400.018.2782462
GTATCAA1196650.018.2155191
TACTTTT674550.017.35255215
Produced by FastQC (version 0.11.5)