FastQCFastQC Report
Thu 26 May 2016
ERR489011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16838366
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236050.14018581137861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178020.10572284745443829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG303150.033.163362
GTACCTG307000.033.09141
ACCTGGG340300.028.7218553
CCTGGGG263800.022.4937654
TATCACG11200.022.4869942
TATAACG16150.020.7217182
TACACGG34600.019.5436882
GTACCCG35200.019.3651581
TACCCGG33550.019.229842
GTACACG40150.018.3565711
GAGTACT587050.018.27593612
AGTACTT598350.017.93650213
ATAACGC21650.017.6885783
GTACATG710050.017.494561
AGAGTAC1032700.017.40101211
GTATCAA1176900.017.17591
GTATAAC29750.017.0973131
GTACTTT629100.016.92816714
TACATGG728100.016.8072512
TAACGCA20300.016.484824
Produced by FastQC (version 0.11.5)