FastQCFastQC Report
Thu 26 May 2016
ERR489010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10158543
Sequences flagged as poor quality0
Sequence length75
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240560.2368056127734066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182490.17964190337137914No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124930.12298023446866346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115580.11377615864794784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT8100.025.98908264
TACCTGG98200.025.9482152
GTACCTG109800.023.9689831
TAGATCG10750.022.4763935
CTCGATA9000.022.24105865
GAGTACT476650.021.40819212
AGTACTT491000.020.89551213
ACCTGGG119500.020.800993
GTACTTT497450.020.29955314
GTACATG775600.019.8918461
GTATCAA1049400.019.3490371
TACATGG788800.019.2596532
AGAGTAC954450.019.11687911
ACATGGG782550.018.8027213
ACTTTTT556600.018.48812916
TGCGACG6750.018.39264322
CATGGGT91600.017.9791134
AGATCGA13800.017.9789226
TACTTTT565500.017.91295615
CATTCCG16200.017.8805779
Produced by FastQC (version 0.11.5)