FastQCFastQC Report
Thu 26 May 2016
ERR489010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10158543
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229070.22549493564185336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180990.17816531366752103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119040.11718215889818058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118600.1167490259183822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG98600.027.5420362
TAGATCG11800.024.5621055
GTACCTG112750.024.5405481
ACCTGGG122250.021.9025153
GAGTACT473350.020.69815312
AGTACTT490800.020.145113
AGATCGA14900.019.9107936
ATGCGAC6450.019.7883621
GTACTTT493200.019.69732914
GTACATG774150.019.6668911
TAGTCGG15250.019.45344240
TGCGACG6800.019.27713622
AATGCGA6700.019.0499920
TACATGG789950.019.0102352
GTATCAA1027600.018.8349481
GCTCGAT9000.018.79045964
ACATGGG782950.018.6197473
AGAGTAC962200.018.25296211
GGTATCA757200.018.0653631
CGCGCCA15700.018.01698152
Produced by FastQC (version 0.11.5)