FastQCFastQC Report
Thu 26 May 2016
ERR489009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19642347
Sequences flagged as poor quality0
Sequence length75
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA420850.2142564735263052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201370.10251829885705613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT565100.020.92091612
GTACCTG207900.020.498021
AGTACTT581700.020.37823313
GTATCAA1148300.019.8231621
GTACATG893900.019.539551
GTACTTT607450.019.45150814
AGAGTAC1097500.018.88917411
TACCTGG218900.018.824352
TACATGG916400.018.7602182
ACATGGG926350.018.0419043
TACTTTT656850.017.9671915
ACTTTTT675550.017.70580916
TATCAAC1331300.016.9885582
TCAACGC1328300.016.967244
ATCAACG1336450.016.8700123
CAACGCA1340950.016.8093575
GTATAGG38100.016.6819931
GGTATCA836550.016.5488281
AACGCAG1377700.016.3852046
ACCTGGG247250.016.0909613
Produced by FastQC (version 0.11.5)