FastQCFastQC Report
Thu 26 May 2016
ERR489009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19642347
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA447500.2278240986171357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200060.10185137244546184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG225400.020.3837241
GAGTACT589050.019.97148912
AGTACTT604200.019.43074413
TACCTGG231850.019.271532
GTATCAA1176000.018.9489571
GTACTTT616150.018.89711614
GTACATG903600.018.7037091
AGAGTAC1137600.018.0684711
TACATGG925700.017.9837652
TACTTTT666100.017.42714315
ACTTTTT677250.017.2370116
ACATGGG937850.017.2173373
GGTATCA873100.016.8646371
ACCTGGG256400.016.3901443
TCAACGC1361000.016.2341334
ACCGTCG15750.016.2093248
ATCAACG1370700.016.114873
TATCAAC1377650.016.0986882
CAACGCA1375050.016.0683365
AACGCAG1410700.015.69895656
Produced by FastQC (version 0.11.5)