FastQCFastQC Report
Thu 26 May 2016
ERR489008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14818180
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230460.15552517245707637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161820.10920369438082139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG256600.036.0426372
GTACCTG260800.035.9643441
ACCTGGG290450.031.1231123
GGTATCA405300.029.3651371
GTATCAA656250.026.9666211
TATAACG13050.025.9636022
CCTGGGG225300.024.7310264
TATCAAC803150.021.8210162
TCAACGC809900.021.633624
ATCAACG815700.021.4777533
TACACGG30100.021.3646532
CAACGCA826400.021.1310065
GTACACG35150.020.9654661
AACGCAG857250.020.5258876
TAACGCA18700.020.1475934
GAGTACT508500.019.84203112
TATCACG8100.019.6346052
GTACATG642450.019.6025351
AGTACTT523150.019.28012313
TACATGG650000.019.1539842
Produced by FastQC (version 0.11.5)