FastQCFastQC Report
Thu 26 May 2016
ERR489008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14818180
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225890.1524411229989108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171320.1156147381122378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG261950.034.8848042
GTACCTG266600.034.8554341
ACCTGGG297250.029.965013
GGTATCA418100.029.330641
GTATCAA668750.025.9598941
TATAACG11400.025.7209362
CCTGGGG232850.023.9410764
TATCAAC832200.020.7840082
ATCAACG835200.020.6312263
TCAACGC835750.020.6299614
ATAACGC17650.020.5222343
CAACGCA850850.020.2957975
AACGCAG881600.019.7091856
GAGTACT526500.019.53630412
TACACGG32050.019.3739052
AGTACTT542850.018.89706213
TACCCGG32850.018.5870552
GTACATG637950.018.5071641
GTACACG37700.018.4381661
TACCGGG19400.018.1372382
Produced by FastQC (version 0.11.5)