FastQCFastQC Report
Thu 26 May 2016
ERR489007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236698
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248790.12933092779228533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG233350.026.1716652
GTACCTG245950.025.440321
ACCTGGG276900.021.1283363
GTACATG917850.020.6471611
GAGTACT655500.020.37408312
TACATGG929500.020.0533922
AGTACTT663900.020.03951613
GAGTACG31350.019.5283951
GTACTTT693600.019.1549614
ACATGGG952750.018.9515973
AGAGTAC1188350.018.65565511
TACTTTT738050.017.9966115
TATAACG13300.017.420942
ACTTTTT775700.017.42086816
TACCCGG27800.017.0421472
CATGGGG544150.016.8942324
CCTGGGG216000.016.5983054
GTACCCG31450.015.9469691
TACGCAG30750.015.8530454
GTATAGG38700.015.8195121
Produced by FastQC (version 0.11.5)