FastQCFastQC Report
Thu 26 May 2016
ERR489007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19236698
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1648220.8568102488275275No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA371980.19336998480716389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232890.12106547599801173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG231250.024.7352872
GTACCTG248350.023.6758821
ACCTGGG273650.020.108543
GTACATG947900.019.6869451
TACATGG967400.019.0115152
GAGTACT675900.018.8795812
AGTACTT685550.018.58392313
GAGTACG29450.018.5782341
ACATGGG980850.018.12833
GTACTTT711400.017.81165514
AGAGTAC1240050.017.31242211
TACTTTT754450.016.79960615
ACTTTTT785000.016.2644716
CATGGGG559150.016.1036834
CCTGGGG207700.015.9126334
TACCCGG25950.015.5547372
TATCACG9700.015.293642
TCTATAC32500.014.8614093
TACGCAG30650.014.7453084
CATGGGT152900.014.3954824
Produced by FastQC (version 0.11.5)