FastQCFastQC Report
Thu 26 May 2016
ERR489005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878529
Sequences flagged as poor quality0
Sequence length75
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA1683221.417027310368144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237320.19978904795366495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184470.1552970068936987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG129150.026.0891271
TACCTGG128950.025.5474072
GAGTACG24300.021.7811181
ACCTGGG153700.020.7561043
GAGTACT471550.020.50992612
GTACGCA23100.020.1994633
AGTACTT485900.019.89728213
AGTACGC24900.019.4358712
GTACTTT496100.019.32359714
TACCCGG13650.018.486962
TACGCAG25550.018.3960844
TACTTTT547450.017.5733515
GTACATG759300.017.5450731
ACTTTTT559700.017.51513516
AGAGTAC1007150.017.34540411
ACGCAGT26800.017.1525025
TACATGG769400.017.0863552
GTACCCG15400.017.0721381
TATAACG7500.017.0535872
TATCACG6800.016.7756862
Produced by FastQC (version 0.11.5)