FastQCFastQC Report
Thu 26 May 2016
ERR489005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489005_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878529
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA316580.2665144817173911No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232220.19549558703775527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196230.16519722265273756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG127000.025.5107631
TACCTGG129050.024.7797642
GAGTACT490600.020.46696912
ACCTGGG153200.020.2433243
TAGATCG6050.019.9565015
AGTACTT504550.019.9557813
GTACTTT508350.019.5555514
GAGTACG21650.019.1650941
TATAACG8650.018.3449752
GTACGCA20550.018.1297933
TACTTTT561850.017.705515
ACTTTTT569800.017.61586216
AGTACGC23250.017.2111722
AGAGTAC1047100.017.02108811
GTACATG764550.016.6338751
TACGCAG22500.016.4050854
ACGCAGT21450.016.4038055
TACATGG778250.016.2232322
GTACCCG14550.015.9220651
ACATGGG776950.015.6955753
Produced by FastQC (version 0.11.5)