FastQCFastQC Report
Thu 26 May 2016
ERR489004_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489004_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15005723
Sequences flagged as poor quality0
Sequence length75
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245340.16349762020796998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171800.11448965171488239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG293850.039.117672
GTACCTG298750.039.0357251
ACCTGGG329400.034.124993
GGTATCA330800.029.8830951
GTATCAA563700.028.8739191
CCTGGGG259200.026.3107514
TATAACG13750.025.8965282
TACACGG29650.025.7676372
GTACACG36950.024.1340581
GTACCCG33700.023.8974251
TATCAAC700150.022.9351542
TCAACGC705100.022.6922554
ATCAACG709250.022.5450823
CAACGCA716550.022.3403535
AACGCAG746300.021.6891736
TACCCGG35700.021.4976882
ATAACGC18750.021.2003063
GTACATG537200.019.7205751
GAGTACT474500.019.68084112
TAACGCA19100.019.5448134
Produced by FastQC (version 0.11.5)