FastQCFastQC Report
Thu 26 May 2016
ERR489004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489004_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15005723
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234470.15625371733171406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179220.11943443178312702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG303450.038.4633372
GTACCTG306700.038.4548451
ACCTGGG331050.034.0786133
GGTATCA362650.029.4024011
TATAACG13850.026.405122
GTATCAA574450.026.2104511
CCTGGGG266400.026.030144
ATAACGC19250.021.5069223
TAACGCA18050.020.642454
TATCAAC728050.020.5712492
TCAACGC728500.020.491424
ATCAACG732600.020.3538043
CAACGCA744500.020.041645
TATCACG11950.019.9210992
GTACCCG32450.019.7239951
GTATAAC26100.019.6181961
AACGCAG775850.019.409876
TACACGG30600.018.9417442
GTACACG36850.018.5894071
GAGTACT499000.018.25177412
Produced by FastQC (version 0.11.5)