FastQCFastQC Report
Thu 26 May 2016
ERR489003_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489003_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14672221
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5157213.514948418511417No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA1215870.8286884446465195No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG1128310.7690110447491215No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA934950.6372245892424876No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG589040.40146614476431347No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA230130.15684741935116708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228020.1554093275994139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160930.10968346237423768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG51800.027.3543661
AGTACGC52750.026.1727142
GTACCTG123200.025.3140681
GTACGCA54550.025.0524233
TACCTGG125400.024.1715722
GCTTATG49250.023.8345381
ACGCAGT61800.021.8241795
TACGCAG62850.021.7456384
GAGTACT451450.020.8269212
AGTACTT455750.020.6235613
ACCTGGG148400.020.0265753
GTACTTT470250.019.82653414
CGCAGTG69800.019.3570166
GTACCCG15950.019.1610031
TACTTTT516000.018.162915
CTTATGT61450.018.0751342
GTACATG626150.018.0371721
AGAGTAC882400.017.92656111
ACTTTTT531700.017.7191716
TACCCGG16650.017.664572
Produced by FastQC (version 0.11.5)