FastQCFastQC Report
Thu 26 May 2016
ERR489003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489003_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14672221
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5231643.5656769346644928No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA983100.670041706705481No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC906540.6178614675992136No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG455860.310695974385882No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG257820.17571981774265805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223960.15264219370741486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167310.11403181563309332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG129100.025.6729681
GAGTACG50900.024.8771231
TACCTGG130850.024.8389992
AGTACGC51550.023.6266942
GTACGCA51400.023.4942673
GCTTATG50950.022.6797941
TACGCAG57400.021.0980974
ACCTGGG153400.020.6478213
ACGCAGT59100.020.4329035
GAGTACT457300.019.61450412
AGTACTT457800.019.57800713
GTACTTT469850.018.89968114
CGCAGTG64900.018.5536886
GTACATG629450.017.813721
GTACCCG15250.017.6954481
TACATGG641700.017.2380622
TACTTTT517050.017.19995915
CTTATGT64000.017.1975922
ACTTTTT530800.016.86488716
AGAGTAC902650.016.7860911
Produced by FastQC (version 0.11.5)