FastQCFastQC Report
Thu 26 May 2016
ERR489002_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489002_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11544623
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA2163611.8741278948649946No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC369650.3201923527515797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293810.2544994323331303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212960.18446682927627867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167220.14484665285302084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143950.1246900829936153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG129950.026.3597581
TACCTGG130800.025.5012782
ACCTGGG146500.022.0301043
AGTACTT476650.020.92905613
GAGTACT481850.020.83825112
GAGTACG26300.020.5858781
GTACTTT503850.019.74420414
GTACATG713050.019.1476251
TACGCAG24500.018.8980714
TACATGG714750.018.7734362
TACTTTT533800.018.62466215
ACTTTTT552250.018.21395116
TAGATCG6900.018.0253895
ACATGGG718100.017.9919343
TATAACG7200.017.7628082
AGAGTAC989000.017.73147211
GTACGCA27250.017.1188493
GTGTAGC51500.017.1169761
CATGGGG385000.016.8274884
ACGCAGT28400.016.0578275
Produced by FastQC (version 0.11.5)