FastQCFastQC Report
Thu 26 May 2016
ERR489002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489002_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11544623
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294170.2548112658161293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220540.19103265650164583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162700.1409314102331449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143420.12423099481031126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG130850.025.144471
TACCTGG131350.024.875112
TAGATCG8000.021.9938285
ACCTGGG150500.021.1826653
AGTACTT475450.020.0259413
GAGTACT478800.019.9074412
GAGTACG27250.019.5519451
GTACTTT492950.019.14005514
GTACATG700050.018.7303491
TACATGG700650.018.4759522
ACATGGG700150.017.8436033
TACTTTT530300.017.7912915
TACGCAG25300.017.7273354
ACTTTTT541600.017.44549216
CATGGGT93550.017.2592814
GTGTAGC57050.016.7980861
GTACGCA27150.016.7744853
AGAGTAC994050.016.64754511
CATGGGG380700.016.221494
GTACCCG17100.015.9833091
Produced by FastQC (version 0.11.5)