FastQCFastQC Report
Thu 26 May 2016
ERR489001_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489001_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21055543
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365040.17337002422592473No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA316470.15030246429645627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243110.1154612825705801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG260050.024.9939382
GTACCTG275900.024.0677381
GTACCCG27750.021.4252091
GAGTACT821200.021.07903912
ACCTGGG300450.020.7329443
AGTACTT844600.020.56537813
TACCCGG27900.019.9505732
GTACTTT863000.019.85171514
GTACATG1206800.019.7983071
AGAGTAC1529900.019.63241811
TACATGG1229500.019.2139452
ACATGGG1236100.018.583173
TACTTTT937500.018.2772315
ACTTTTT978200.017.91186316
GAGTACG27700.017.3458141
CATGGGT199300.017.2405134
GTATAGG52700.016.5291121
CATGGGG595900.016.1207894
GTGTAGC134100.015.7496911
TATCACG10400.015.6242152
Produced by FastQC (version 0.11.5)