FastQCFastQC Report
Thu 26 May 2016
ERR489001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489001_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21055543
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340270.1616059011159199No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG340000.16147766884948064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247490.11754149489281752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG257550.024.7959062
GTACCTG273800.023.8349841
ACCTGGG297800.020.5639083
GAGTACT846750.019.8903612
AGTACTT870700.019.41046113
GTACTTT882050.018.88314
GTACATG1201300.018.5153121
AGAGTAC1578600.018.02505511
TACATGG1225300.017.8715062
GTACCCG27200.017.428431
TACTTTT956950.017.38307615
ACATGGG1229700.017.3024183
ACTTTTT989350.017.00209616
GAGTACG29300.016.769781
TACCCGG27100.016.5504252
TATAACG18800.016.3330482
TAGATCG12500.016.283715
CATGGGT191600.016.2053874
TAGCCCT112750.015.0543154
GTGTAGC138600.014.9544431
Produced by FastQC (version 0.11.5)