FastQCFastQC Report
Thu 26 May 2016
ERR489000_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489000_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13659851
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202400.14817145516448166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142110.104034809750121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG268450.038.239151
TACCTGG265100.038.211322
GGTATCA263700.037.2616651
ACCTGGG286450.034.609253
GTATCAA484600.031.4568441
CCTGGGG217400.026.885634
TATAACG13250.026.0926552
TATCAAC610900.024.8047872
TCAACGC616750.024.4630854
ATCAACG618050.024.401893
GTACACG30050.024.063341
TACACGG26850.023.949852
CAACGCA632550.023.8517745
GTACCCG27500.023.4009951
AACGCAG660150.022.993186
TATCACG10900.022.2026962
ATAACGC16800.021.1928733
TACCCGG29200.020.6015822
TAACGCA17400.020.460874
ACGCAGA745850.020.2570657
Produced by FastQC (version 0.11.5)