FastQCFastQC Report
Thu 26 May 2016
ERR489000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR489000_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13659851
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192360.1408214481988127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145670.10664098751882432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG277650.039.0869332
GTACCTG283850.038.633171
GGTATCA262750.036.7999651
ACCTGGG307000.034.575943
GTATCAA477350.030.637531
CCTGGGG221700.028.413084
TATCAAC610250.023.8717272
TCAACGC613800.023.6669124
TAACGCA15600.023.6615094
ATCAACG614350.023.6401983
TATAACG14900.023.614812
CAACGCA627150.023.1841035
TATCACG9900.022.9974292
AACGCAG654850.022.3825266
TACCCGG28450.021.5828232
GTACCCG28950.021.382451
GTACACG29300.020.7729421
ATAACGC18900.020.6253573
TACACGG27050.019.8942642
ACGCAGA750200.019.4862127
Produced by FastQC (version 0.11.5)